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KMID : 0371320080750050287
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Journal of the Korean Surgical Society
2008 Volume.75 No. 5 p.287 ~ p.295
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A Pig to Canine Auxiliary Hepatic Xenotransplantation Model - Prevention of Hyperacute Rejection via Blocking the Kupffer Cells and Regulating the Complement Family -
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Park Jae-Jeong
Chung Ku-Yong
Lee Jeong-Eun
Yom Cha-Kyong
Lee Jae-Gil
Ahn Hyung-Joon
Oh Sei-Kwan
Sung Sun-Hee
Kang Byung-Chul
Han Ki-Hwan
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Abstract
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Purpose: We designed a pig to canine liver xenotransplantation model to study the diverse immunologic and hemodynamic consequences that follow xenotransplantation and hyperacute rejection.
Methods: The animals were divided into two groups: the cobra venom factor and Gadolinium chloride treatment group (CVF＋Gd group) (3 cases) and the control group (3 cases). The donor pig’s whole liver was harvested, and then the harvested pig’s whole liver was transplanted into a dog after the dog underwent left hepatectomy. After reperfusion of the graft, blood samples were taken 20, 40 and 60 minutes after reperfusion, and the liver, lung and kidney tissues were taken 1 hour after reperfusion.
Results: In the control group, the grafts showed a patchy hypoperfused liver surface and it felt rubbery solid compared to the CVF＋Gd group. The serum total protein, albumin, fibrinogen and platelets decreased abruptly and there were no significant differences between the two groups. The serum PT, PTT and FDP were increased in both groups and the CVF＋Gd group showed a more obtuse slope than the control group. We could not find any intravascular pathologic changes on the microscopic findings of the graft. Only scant intravascular fibrin deposition was found. Hepatocellular vacuolization and sinusoidal dilatation were also found. There were patches of necrosis without any zonal distribution, intrasinusoidal neutrophil sequestration and interstitial hemorrhage. These findings were milder in the CVF＋Gd group.
Conclusion: The pig to canine partial auxiliary liver xenotransplantation model is feasible and it is a good model before starting to perform pig to primate liver xenotransplantation. In the CVF＋Gd group, pathologic findings like patch hepatocyte necrosis etc. were less severe. As there were no corresponding vascular pathologic findings, these findings are not the direct effect of CVF and gadolinium treatment, and so other factors like Ischemia- reperfusion injury should be considered. (J Korean Surg Soc 2008;75:287-295)
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KEYWORD
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Xeno-liver transplantation, Pig to canine, Cobra venom factor
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